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Determination of pH of Wastewater Sample

In this determination of pH of wastewater sample post we have briefly explained about determination of pH of waste water sample principle, sample preparation, reagents, procedure, and result.

Determination of pH of Waste Water Sample

On a scale of 1.0 to 14.0, pH is a measurement of the hydrogen ion concentration in water. The lower the pH, the more acidic the water. The more basic, or alkaline, water is, the higher its pH. pH has an impact on many chemical and biological processes in water, and various organisms thrive in different pH ranges.

A pH range of 6.5 to 8.0 is preferred by the majority of aquatic organisms. Because it affects the physiological systems of most species and can inhibit reproduction, pH outside of this range diminishes stream variety. Toxic elements and compounds, such as heavy metals, can become mobile and “accessible” for uptake by aquatic plants and animals when the pH is low.

Due to variations in water temperature, water velocity, and the chemical makeup of the chemicals present in the water, direct measurement of the pH level of wastewater effluent can fluctuate substantially. As a result, precise pH testing of wastewater also necessitates temperature monitoring of the water.

Standard pH electrodes can be destroyed by chemical and particle contamination in wastewater, prematurely destroying the connection. For wastewater testing, specific pH electrodes are recommended.


A pH metre is precision equipment that measures the movement of hydrogen ions in water based solutions and displays the acidity or alkalinity as pH.

The word pH is made up of the letters “p,” which stands for negative logarithm, and “H,” which stands for hydrogen. The level of acidity or alkalinity of a suspension is expressed in pH, which is a unit of measurement. It is rated from 0 to 14 on a scale of 1 to 14. pH = –log [H+] for determination of pH of Waste Water Sample.

Sample Collection

Fill a sealed container with two to three representative samples from each site for determination of pH of waste water sample. Take hold of samples of wastewater will off-gas the volatile chemicals present, causing the pH level of the sample to change; consequently, samples must be collected into a sealed container.

There should be no time between taking the sample and testing the pH value. There is no need for sample preservation or preparation for determination of pH of waste water sample.

Required Reagents

1. pH-Meter

2. Buffers (4.01 and 7.00)

3. Distilled water

4. Glass Beaker

5. Magnetic Stirrer

6. Stir Bar

7. Tissue paper


1. Collect and transfer waste water from the polluted lake into a beaker. Turn the pH meter on. Rinse the electrodes in water after removing them from the storage solution.

2. Tissue paper soaked in water is used to bloat. Standardize the instrument by immersing the electrodes in a buffer solution (pH = 4 acetate buffer) for correct determination of pH of waste water sample.

3. Use a pH metre to determine the pH (pH = 7). The electrodes should be rinsed, bloated, and dried. Standardize the equipment by immersing the electrodes in a buffer solution (pH = 10 ammonium buffer). Rinse, bloat, and dry the electrodes, then use a pH metre to check the pH (pH = 7).

4. Dip the pH electrode into the waste water to be tested beaker. Take note of the pH. Rinse the electrodes in water after measurement. Replace the electrodes in the storage solution of next determination of pH of waste water sample analysis.


Skin and organ linings will be irreversibly damaged if the pH falls below 2.5. Lower pH values increase the danger of mobilised harmful metals being absorbed, even by people, and pH levels above 8.0 can’t be cleaned properly with chlorine, posing extra concerns indirectly. Furthermore, pH levels outside of 6.5-9.5 can corrode and destroy pipelines, increasing heavy metal toxicity even more.

Further Readings


Determination of pH of Wastewater Sample