Separation of Plasma and Serum from Whole Blood

In this separation of plasma and serum from whole blood post we have briefly explained about blood serum, blood plasma, difference between plasma and serum, separation of plasma and serum.

Blood is a fluid connective tissue that transports essential nutrients, oxygen, and metabolic wastes throughout the body. It also interacts with acids and bases to regulate the body’s temperature and pH levels.

RBCs (Red Blood Cells), WBCs (White Blood Cells), plasma, and blood sera make up the majority of blood. The primary components of blood are plasma and serum, which are regularly utilised in blood group test procedures to determine the patient’s blood group.

Blood Serum

After clotting factors (such as fibrinogen and prothrombin) have been eliminated by clot formation, the clear yellowish fluid that remains from blood plasma.

Blood Plasma

Plasma takes up around 55% of the total volume of blood. It is made up of 90% water and is the liquid element of blood. Apart from water, plasma also contains fibrinogen (which aids in regular blood clotting) and albumin (which acts to keep fluid in your bloodstream and prevent leaking into other tissues). Plasma in the blood is responsible for transporting proteins, nutrients, antibodies, hormones, and other substances throughout the body.

Plasma and Serum Isolation

Separation of Plasma and Serum from Whole Blood, Image Source : https://owlcation.com/stem/difference-between-plasma-and-serum

Difference between Plasma and Serum

The liquid fraction of the blood collected after the cells are removed is used to make serum and plasma. Plasma and serum, on the other hand, have a significant difference. The liquid that remains after blood clots is known as serum. Plasma, on the other hand, is the liquid that remains after an anticoagulant has been injected to prevent clotting.

Serum

Plasma

The extracellular part of blood is an undiluted fluid.

A liquid part of the blood that is translucent and straw-colored.

After coagulation, serum is the liquid portion of the blood.

Plasma is a transparent, yellowish fluid that makes up a portion of the blood.

Serum and clotting factor make up this mixture.

It’s the part of the blood that doesn’t have any clotting factor.

After centrifuging coagulated blood, it is obtained.

After centrifuging blood with an anticoagulant, it is obtained.

In comparison to plasma, serum has a smaller volume.

Plasma is a transparent yellow liquid that makes up 55 percent of total blood volume.

After clotting, serum is obtained through the spinning process.

Before clotting, plasma is obtained through the spinning process.

It’s difficult to separate serum from blood. This is a lengthy procedure.

Separating the plasma from the blood sample is quite simple and takes little time.

Serum contains antibodies that react with the antigen of the recipient.

Antibodies, a type of protein, are found in blood plasma and can fight substances that are alien to the host body.

Contains 90% water, as well as dissolved hormones, proteins, minerals, and carbon dioxide.

Proteins, salts, lipids, and carbohydrates make up 92 percent of the composition.

The density of serum is around 1.024 g/ml.

The density of plasma is roughly 1025 kg/m3, or 1.025 g/ml.

It has a limited shelf life. It can only be kept for a few months.

The product has a lengthy shelf life. It can last for up to ten years if properly stored.

Animal sera are utilised as anti-venom, anti-toxins, and vaccines, and serum is a vital supply of electrolytes.

Plasma is made up of proteins that aid in the transfer of materials like glucose and other dissolved nutrients through the bloodstream.

Separation of Plasma and Serum

Serum isolation

Allow the blood to clot by leaving it undisturbed at room temperature after collecting the whole blood. It normally takes 15–30 minutes to do this task. In a chilled centrifuge, remove the clot by centrifuging it at 1,000 – 5,000 x g for 10 minutes. Sera is the name given to the supernatant those results.

Plasma isolation

Collect whole blood in anticoagulant-treated tubes from the pharmacy, such as EDTA-treated (lavender caps) or citrate-treated tubes (light blue tops). Using a chilled centrifuge, cells are separated from plasma by centrifugation for 10 minutes at 1,000–2,000 x g. Platelets in plasma are depleted by centrifugation at 2,000 x g for 15 minutes.

Further Readings

Reference