Home » BOD Incubator Definition, Principle, Components, Application

BOD Incubator Definition, Principle, Components, Application

Biological Oxygen Demand Incubator or BOD Incubator are pieces of microbiological testing equipment that are routinely used to maintain and grow cell and microbiological cultures. It is used to regulate temperature for bacterial culture storage, test tissue culture development, and incubation where a high degree of continuous temperature precision is required.

BOD Incubator Definition

Bio-Oxygen Demand incubator is a specialised type of incubator designed to maintain a constant temperature of 20 degrees Celsius. This incubator is utilised by several sectors to establish similar conditions. Some companies use it to determine the ideal BOD level at a certain temperature for their products. In laboratories, BOD incubators are also employed to examine germination, insects, and bacterial cultivation.

Biochemical oxygen demand and biological oxygen demand are other names for it. In BOD incubator oxygen depletion is caused when microorganisms are initiated to consume oxygen. They use oxygen as an electron receptor. When microbes intake organic material it provides them energy to survive and multiply. Similarly, the oxidation method is proceeding without the assistance of the microorganisms. This whole thing is estimated by the COD method i.e. chemical oxygen demand.

BOD incubator Principle

The BOD Incubator is intended to achieve the optimal development of for biochemical processes. The Instrument operates on the basis of thermo-temperature. It comprises of a thermostat to raise the temperature, a biosensor to detect the rise in temperature, and a PID-controlled cooling system. Temperature is set through digital PID temperature controller, usually at 20°C. Utilizing a control loop feedback mechanism, the PID controller efficiently maintains the temperature.

BOD incubator Diagram

BOD incubator Diagram

Figure 1: BOD incubator Diagram

Parts of BOD Incubator

  1. Body Cabinet: The body of the cabinet is composed of stainless steel. The cabinet is equipped with an insulator that prevents heat from escaping, therefore maintaining the temperature.
  2. Body Shelves: The cabinet area is partitioned by shelves, which give room for microbial culture placement.
  3. Control system: A temperature control system makes sure that the biological oxygen demand incubator stays at a constant 20°C temperature.
  4. O2 monitoring: The amount of oxygen in the biological oxygen demand incubator chamber is measured by an oxygen monitoring system.
  5. Shaking mechanism: This helps to ensure that the microorganisms in the sample are evenly distributed and that they have access to oxygen.
  6. A sample chamber: This is where the water sample is placed for the duration of the BOD test.
  7. A light source: This is used to provide the necessary light for photosynthesis in the BOD test sample.
  8. An air pump: This pumps air into the BOD test incubator chamber to maintain the necessary oxygen levels.
  9. A data logger: This records the oxygen concentration in the BOD test incubator chamber over the course of the BOD test.
  10. A control panel: This allows the operator to set and monitor the various parameters of the BOD test.

Procedure of BOD Incubator

  1. First, you must ensure that the Biological Oxygen Demand incubator is plugged into the power source before turning it on.
  2. Then, power the system by turning on the Biological Oxygen Demand incubator cabinet’s power switch and the mainboard’s power switch.
  3. Then, using the controller’s set knob and soft key, you may input in the required temperature. Maintain a consistent temperature throughout the day by following these steps.
  4. Take note of the controller’s current temperature reading. Pay attention to the digital temperature reading. A difference of more than 2 degrees Celsius is unacceptable.

Application of BOD incubators

The Major applications of BOD Incubators are; 

  1. The pharmaceutical, agricultural, beverage, and laboratory sectors are just some of the many users of these Biological Oxygen Demand incubator.
  2. Manufacturers use Biological Oxygen Demand incubator in waste treatment facilities to characterize treatment system efficiency.
  3. The Biological Oxygen Demand incubator is used in alcoholic beverages in order to differentiate the nourishment under intended working circumstances.
  4. Biological Oxygen Demand incubator is used in farming to define the germination of anaerobic bacteria (bacteria that don’t need oxygen).

Advantages of BOD Incubator

A biological oxygen demand (BOD) incubator is a piece of specialised lab equipment used to quantify the quantity of oxygen consumed by microorganisms during the decomposition of organic waste. It is widely utilised in water quality testing and environmental research facilities. Biological Oxygen Demand incubator may help you in several ways:

  1. Precision: The amount of organic matter in water may be inferred from the oxygen consumption rate of microbes, which is precisely measured by this method.
  2. Real-Time: In biological oxygen demand incubator, oxygen use may be tracked in real time and over time for more precise findings (biological oxygen demand test).
  3. Accuracy: This method of BOD measurement yields accurate and repeatable findings that are in line with those obtained using gold-standard laboratory techniques.
  4. User Friendly: Minimal instruction is needed, and it’s quite user-friendly. It may be used in a wide range of institutions, from research labs to wastewater treatment plants.

Disadvantages of BOD Incubator

Here are some potential disadvantages of using a Biological Oxygen Demand incubator:

  1. High Cost: Biological Oxygen Demand incubator can be expensive to purchase and maintain, especially if you need a high-end model with advanced features.
  2. The Size: Biological Oxygen Demand incubator are typically large and bulky, which can be a disadvantage if you have limited space in your laboratory.
  3. Maintenance: Like any other laboratory equipment, BOD incubators require regular cleaning and maintenance to ensure that they are functioning properly. This can be time-consuming and may require specialized training.
  4. Safety: BOD incubators contain microorganisms that may pose a risk to human health if they are not handled properly. It is important to follow proper safety protocols when working with these incubators to prevent the spread of infection.
  5. Accuracy: BOD incubators rely on temperature and humidity controls to maintain the proper conditions for growth. If these controls are not calibrated properly, the incubator may not function as intended, leading to inaccuracies in your results.

Precautions Using Incubator

When not in use, never leave the BOD incubator plugged in. Regular maintenance is essential for keeping the incubator in good operating order and preventing any unplanned downtime. Always refer to the manufacturer’s guidelines before using a Biological Oxygen Demand incubator. The incubator must be unplugged from its power source before any cleaning can begin. Be sure to keep the Biological Oxygen Demand incubator clean on a regular basis to keep it running well. Check the BOD incubator’s temperature once a month.


FAQs on BOD Incubator

Bio-Oxygen Demand incubator is a specialised type of incubator designed to maintain a constant temperature of 20 degrees Celsius.

Incubating the samples in the dark stops photosynthesis from making more oxygen in the samples.

BOD incubators are named after the biological oxygen demand (BOD) of microorganisms. BOD is a measure of the amount of oxygen that microorganisms consume during the process of decomposition