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Multiple Choice Questions on Cloning Vectors
Cloning vector is a DNA molecule that transports foreign DNA into the host cell. It is capable of self-replication and integration into the host cell.
MCQ on Cloning Vectors
1. Vectors designed to replicate in cells of two different species are known as
- Phasmids
- Transfer vectors
- Shuttle vectors
- Phagemids
2. Autonomously replicating sequences (ARS) is a characteristic feature of
- Plasmid vectors
- Phage vectors
- E.coli vectors
- Yeast vectors
3. Which of the following are vectors for animals?
- SV 40 vectors and Bovine papillomavirus vectors
- CMV vectors and Gemini vectors
- Lambda phage and M13 phage vectors
- All of the above
4. Maximum size of foreign DNA that can be inserted into a replacement vector is
- 25-30 kb
- 18-20 kb
- 20-25 kb
- 40-50 kb
5. Select the wrong statement about plasmids?
- It is extrachromosomal
- It is double stranded
- Its replication depends upon host cell
- It is closed and circular DNA
6. P1 cloning vector allow cloning of DNA of the length of
- 100 kbp
- 50 kbp
- 20 kbp
- 10 kbp
7. Size of the DNA that can be packaged into a λ phage is
- 50 kb
- 35-53 kb
- 40-50 kb
- Any size
8. Cosmid vectors are used for
- Cloning small fragments of DNA
- Cloning large fragments of DNA
- Cloning prokaryotic DNA only
- Cloning eukaryotic DNA only
9. Cryptic plasmids
- Do not exhibit any phenotypic trait
- Exhibit many phenotypic traits
- Exhibit one phenotypic traits
- Exhibit antibiotic resistance
10. Maximum size of foreign DNA that can be inserted into an insertion vector is
- 35 kb
- 18 kb
- 50 kb
- 27 kb
11. Cosmids lack
- Genes coding for viral proteins
- Origin of replication
- Marker genes coding for replication
- Cleavage site for the insertion of foreign DNA
12. Single stranded vectors are useful
- For sequencing of cloned DNA
- For oligonucleotide directed mutagenesis
- For probe preparation
- All of the above
13. Stuffer is
- The right arm of the vector DNA
- The left arm of the vector DNA
- Central fragment of the vector DNA
- None of the above
14. λ ZAP vector is an example of
- Phage
- Phagemid
- Cosmid
- Plasmid
15. The most common vectors for plants are
- SV 40 vectors and Bovine papillomavirus vectors
- Cauliflower mosaic virus (CMV vectors) and Gemini vectors
- Lambda phage and M13 phage vectors
- T4 phage vectors
Answer Key
1. Shuttle vectors
2. Yeast vectors
3. SV 40 vectors and Bovine papillomavirus vectors
4. 20-25 kb
5. Its replication depends upon host cell
6. 100 kbp
7. 35-53 kb
8. Cloning large fragments of DNA
9. Do not exhibit any phenotypic trait
10. 18 kb
11. Genes coding for viral proteins
12. All of the above
13. Central fragment of the vector DNA
14. Phagemid
15. Cauliflower mosaic virus (CMV vectors) and Gemini vectors
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