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Genetic Engineering MCQ Questions and Answers

Genetic engineering MCQ questions and answers below can help students evaluate their knowledge of concepts. Answers are also given for MCQ on genetic engineering to help you remember. Try our MCQ on genetic engineering to see if you can get all the answers right for the questions below.

Genetic Engineering MCQ Questions and Answers

Genetic engineering is the process of modifying an organism’s genetic makeup using recombinant DNA (rDNA) technology.  The manipulation of one or more genes is referred to as genetic engineering. Typically, a gene from another species is inserted into an organism’s genome to confer the desired phenotype.

MCQ on Genetic Engineering

1. DNA sequencing is carried out by

a. Maxam Gilbert method

b. Sanger dideoxy method

c. Both a and b

d. Watson and Crick

2. DNA staining is done by

a. Cystal violet

b. Giemsa staining

c. Methylene blue

d. Feulgen staining

3. Which of the following is not a restriction endonuclease?

a. Eco RI

b. DNA ligase

c. Hind III

d. Bam H1

4. Use of alkaline phosphatase is to

a. Remove terminal phosphates from 3’end

b. Remove terminal phosphates from 5’end

c. Remove terminal phosphate from both end

d. All of these

5. The uptake of plasmid DNA into bacterial cell is facilitated in the presence of

a. Calcium Chloride

b. Magnesium Chloride

c. Potassium Chloride

d. All of These

6. Taq polymerase is used in PCR because of its

a. Low Thermal Stability

b. High Fidelity

c. High Speed

d. High Thermal Stability

7. Introduction of recombinant DNA into bacterial cell by using current is called

a. Transformation

b. Electroporation

c. Transformation

d. Transduction

8. Nick translation is done by

a. DNA polymerase I

b. DNA polymerase II

c. DNA ligase

d. Kinase

9. Telomeric sequences are found in

a. HAC

b. BAC

c. YAC

d. PAC

10. Which of the following activity is not present in Taq polymerase?

a. 5’-3’ polymerase

b. 5’-3’ exonuclease

c. 3’-5’ exonuclease

d. Both 5’-3’ polymerase and 5’-3’ exonuclease

11. What is the half life cycle for Taq polymerase?

a. 40 minutes

b. 80 minutes

c. 10 minutes

d. 50 minutes

12. Taq polymerase incorporates which residue at 3’ end?

a. G

b. T

c. A

d. C

13. Thermococcus litoralis grows at a temperature up to 98 degrees.

a. True

b. False

14. The first crop plant genome sequenced

a. Maize

b. Wheat

c. Rice

d. Barley

15. Urea and Formamide can?

a. Increase Tm of DNA

b. Decrease the Tm of DNA

c. Helps faster re-annealing of DNA

d. Does not effect denaturation of DNA

Answer Key

Genetic Engineering MCQ

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