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Ion Exchange Chromatography MCQ with Answers

  • Ion Exchange Chromatography MCQ with answers can help students evaluate their knowledge of concepts in Ion Exchange Chromatography. Answers are also given to help you remember. Try our Ion Exchange Chromatography MCQ to see if you can get all the answers right for the questions below ion exchange chromatography is based on the MCQ.

Ion Exchange Chromatography MCQ

  • Ion exchange chromatography (also known as ion chromatography) is a technique that allows ions and polar compounds to be separated based on their affinity for ion exchangers. Ion exchange chromatography is based on the MCQ and answers are given below.

     

1. Which of the following ion get released from the cation exchange column?

  1. H+
  2. Na+
  3. K+
  4. Ca+2

2. Which of the following ion get released from the anion exchange column?

  1. CO3-2
  2. OH–
  3. Cl–
  4. SO4-2

3. Which of the following is not an ion exchange technique?

  1. Batch method
  2. Column method
  3. Paper Chromatography
  4. A and B

4. What is the use of demineralized water?

  1. Biological studies
  2. Conductance experiment
  3. A and B
  4. None of the above

5. Ion-free water coming out from the exchanger is known as ____________

  1. Potable water
  2. Disinfected water
  3. Coagulated water
  4. Demineralized water

6. Protein purification refers to the

  1. Purification of proteins
  2. Separation of proteins from other biomolecules
  3. Separation of a particular protein from other contaminating proteins
  4. All of these

7. In reverse phase chromatography, the stationary phase is made

  1.  Non-polar
  2. Polar
  3. Either non-polar or polar
  4. None of these

8. Which of the following is not an application of ion Exchange?

  1. It is used for softening of water.
  2. It is used for demineralization of water.
  3. It is used for separation of similar ion in one sample.
  4. It is used in preformulation

9. Primary steps in protein purification includes

  1. Homogenization
  2. Differential centrifugation
  3. Solubilisation
  4. All of These

10. The use of insulin hormone to purify its receptor is an example of

  1. Ion exchange chromatography
  2. Affinity chromatography
  3. Gel filtration chromatography
  4. Ligand mediated chromatography

11. Ion-exchange chromatography is used for the separation of

  1. Polar molecules
  2. Non polar molecules
  3. Both of above
  4. None of above

12. Ion exchange chromatography is based on the

  1. Adsorption chromatography
  2. Electrical mobility of ionic species
  3. Electrostatic attraction
  4. Partition chromatography

13. Which of the following statements about the batch method is correct?

  1. It is multiple step process.
  2. It is not used for preparation of the demineralized water.
  3. In this process more than two containers.
  4. This is single step process.

14. The ion exchange capacity of a resin is determined by

  1. The total molecular weight of the resin
  2. Length of the ion exchange resin
  3. The total number of ion active groups
  4. Solubility of the ion exchange resins

15. What methods are used to create demineralized water?

  1. Gas Chromatography
  2. Batch method (ion exchange)
  3. Mass spectroscopy
  4. Complexometric Titration

Answer Key

  1. OH–
  2. Paper Chromatography
  3. A and B
  4. A and B
  5. Demineralized water
  6. Separation of a particular protein from other contaminating proteins
  7. Non-polar
  8. It is used in preformulation
  9. All of These
  10. Affinity chromatography
  11. Polar molecules
  12. Adsorption chromatography
  13. This is single step process.
  14. The total number of ion active groups
  15. Batch method (ion exchange)
  • We hope Ion exchange chromatography is based on the MCQ and concepts are clear for now.

Further Readings

  1. MCQ on Paper Chromatography
  2. Gas Chromatography MCQ
  3. MCQ on Thin Layer Chromatography
  4. MCQ on Ecology
  5. MCQ on Nucleic Acid – RNA

Reference

  1. https://chrominfo.blogspot.com/2020/04/advantages-and-disadvantages-of-ion.html
  2. https://cdn.intechopen.com/pdfs/43603/InTech-Ion_exchange_chromatography_an_overview.pdf
  3. http://wolfson.huji.ac.il/purification/PDF/IonExchange/AMERSHAM_ion_exchangeManual.pdf