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Multiple Choice Questions on DNA Extraction

Multiple Choice Questions on DNA Extraction; can help students evaluate their knowledge of concepts in DNA extraction and methods. Answers are also for given for DNA Extraction MCQ to help you remember. Try our MCQ on DNA Extraction to see if you can get all the answers right for the DNA Extraction MCQ questions given below.

Multiple Choice Questions on DNA Extraction

DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components.

DNA Extraction MCQ

1. Which is the best method for isolating DNA from plants?

a. CTAB method

b. SDS-phenol extraction

c. SDS-proteinase K treatment

d. All of these

2. Which of the following reagents is frequently used to lyse bacterial cell walls?

a. CTAB

b. Phenol extraction

c. Lysozyme

d. Penicillin

3. The extraction of DNA from plant tissues is difficult due to?

a. Presence of large amount of DNA

b. Presence of large amount of RNA along with DNA

c. Both a and b

d. Presence of secondary metabolites and polysaccharides

4. CTAB’s role in DNA extraction from plant tissue is?

a. CTAB binds to nucleic acids and forms a precipitate

b. CTAB binds to proteins and forms a precipitate

c. CTAB binds to polysaccharides and forms a precipitate

d. CTAB binds to secondary metabolites to form a precipitate

5. Which of the following reagents are used for precipitating DNA?

a. Isopropanol

b. Ethanol

c. Both a and b

d. None of these

6. Role of EDTA in EDTA-lysozyme treatment in bacterial DNA extraction is?

a. Removes Mg+ ions essential for maintenance of bacterial cell wall structure

b. Inhibits degradation of DNA by cellular enzymes

c. Removes outer lipopolysaccharide layer of Gram negative bacteria

d. All of these

7. Phenol used in DNA extraction

a. Precipitates DNA and leave proteins in aqueous solution

b. Precipitates RNA-protein complex and leave DNA in aqueous solution

c. Precipitates cell debris and leave nucleic acids-protein complex in aqueous solution

d. Precipitates proteins and leave nucleic acids in aqueous solution

8. Prior to phenol treatment in cell extracts with a high protein content

a. Chloroform is used to break polypeptides to small fragments

b. SDS is used to break polypeptides to small fragments

c. Proteases are used to break polypeptides to small fragments

d. All of these

9. Which of the following reagents is used to measure DNA?

a. Chloroform

b. CTAB

c. Diphenylamine

d. Dansyl chloride

10. Which of the following is correct in terms of DNA extraction order?

a. RNAase treatment->Protease treatment->Cell lysis-> ethanol precipitation

b. Cell lysis->phenol treatment->RNAase treatment-> ethanol precipitation

c. Cell lysis-> RNAase treatment ->protease treatment-> ethanol precipitation

d. Cell lysis-> phenol treatment ->protease treatment-> ethanol precipitation

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MCQ on DNA Extraction

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