MCQ on DNA Sequencing With Answers

MCQ on DNA sequencing with answers article focuses on DNA sequencing Maxam-Gilbert method and Sanger’s method.

MCQ on DNA sequencing

MCQ on DNA sequencing

1. The first DNA sequences were discovered in the early?

a) 1930s

b) 1920s

c) 1970s

d) 1980s

2. DNA sequencing is used in

a) Forensic studies

b) Biotechnology

c) Diagnostics

d) All of above

3. The process of figuring out the exact order of DNA’s nucleotides is

a) DNA replication

b) Denaturation

c) Blotting

d) DNA sequencing

4. DNA sequencing refers to the

a) Method used to figure out the order of sugars in a DNA

b) Method used to figure out the order of phosphates in a DNA

c) Method used to figure out the order of the bases in a DNA

d) These are all

5. Which enzyme used in Maxam-Gilbert method for 32P labelling of the DNA at 3’ end?

a) Polynucleotide kinase

b) Alkaline phosphatase

c) Exonuclease

d) Terminal nucleotidyl transferase

6. Sanger’s method is based on the principle of

a) Chemicals employed for base-specific cleavage

b) The utilisation of dNTPs for chain termination

c) Use of ddNTPs to terminate chains

d) Application to 32P chain termination

7. After the reaction, the samples in Sanger’s method are separated using

a) AGE

b) PAGE

c) PFGE

d) 2-D gel electrophoresis 

8. Which method generates sequence templates using PCR?

a) Sanger’s method

b) Sanger’s method and LMPCR

c) LMPCR

d) LMPCR and automated DNA sequencing

9. Sanger’s method has been improved with automated DNA sequencing, where?

a) ddNTPS are utilised to terminate chains

b) PCR is employed to produce sequencing templates

c) Fluorescently marked dNTPs are employed for chain termination

d) Fluorescently marked ddNTPs are employed for chain termination

10. Which of the following is not a DNA sequencing method?

a) LMPCR

b) Edmans method

c) Sanger’s method

d) Maxam-Gilbert method

MCQ on DNA sequencing with answers

  1. c) 1970s
  2. d) All of above
  3. d) DNA sequencing
  4. c) Method used to figure out the order of the bases in a DNA
  5. a) polynucleotide kinase
  6. c) Use of ddNTPs to terminate chains
  7. b) PAGE
  8. c) LMPCR
  9. d) Fluorescently marked ddNTPs are employed for chain termination
  10. b) Edman’s method

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