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Multiple Choice Questions on Gene Transfer Methods

Multiple Choice Questions on Gene Transfer Methods can help students evaluate their knowledge of concepts on genetic engineering and enzymes used in GE. Answers are also given for MCQ on gene transfer methods to help you remember. Try our MCQ on Gene Transfer Methods to see if you can get all the answers right for the questions below.

Multiple Choice Questions on Gene Transfer Methods

The gene transfer process is frequently employed in both basic and applied biology.  DNA transfer into animal cells is a basic and well-established method. It’s become a must-have tool for gene cloning, gene function and regulation research, and the creation of small batches of recombinant proteins for testing and verification.

MCQ on Gene Transfer Methods

1. Virus-mediated gene transfer using GM bacteriophages is known as?

a. Transfection

b. Transduction

c. Transformation

d. Conjugation

2. Ability of cells to take up DNA fragments from their surroundings is referred to as?

a. Transfection

b. Transduction

c. Transformation

d. Conjugation

3. Which of the following bacteria is a natural genetic engineer?

a. Agrobacterium tumefaciens

b. Agrobacterium radiobactor

c. Psueudomonas putida

d. Thermus aquaticus

4. The removal of cancer-causing genes from the Ti plasmid is known as?

a. Gene replacement

b. Disarming

c. Insertional inactivation

d. Gene displacement

5. Agrobacterium tumefaciens is a?

a. Gram negative soil bacterium causing crown gall disease in dicots

b. Gram negative soil bacterium causing crown gall disease in monocots

c. Gram positive soil bacterium causing crown gall disease in dicots

d. Gram positive soil bacterium causing crown gall disease in dicots

6. Which is about agrobacterium-mediated gene transfer is correct?

a. Virus genes are essential for gene transfer

b. T-DNA borders are essential for gene transfer

c. Both a and b

d. None of these

7. Ti plasmid vectors include?

a. Binary vectors and co-integrate vectors

b. Co-integrate vectors and multiple vectors

c. Multiple vectors and binary vectors

d. Ti plasmid based vector

8. Which of the following chemicals promotes the expression of virus genes?

a. Cyanidin

b. Glutennin

c. Acetosyringone

d. Dextran

9. Among the chemicals used in gene transfer methods are?

a. Poly ethylene glycol

b. CaCl2

c. Dextran

d. All of the above

10. Intro of DNA into cells by exposing to high voltage electric pulse is known as?

a. Electrofusion

b. Electrofision

c. Electrolysis

d. Electroporation

11. Transformation method that employs gold particles coated with DNA and accelerated at high speeds is known as?

a. Acceleration method

b. High-velocity method

c. Particle gun delivery method

d. DNA particle delivery method

12. Method of transforming in-vitro animal cell cultures that use of liposomes?

a. Lipotransformation

b. Liposome mediated transformation

c. Lipofection

d. Lipid mediated DNA transfer

13. DNA solution injected directly into the cell using micromanipulators is known as?

a. Macroinjection

b. Micromanipulator mediated DNA delivery

c. Microfiction

d. Microinjection

14. Fibre mediated DNA delivery uses

a. Silicon carbide fibres that will create pores in the membrane

b. Aluminium carbide fibres that will create pores in the membrane

c. Boron carbide fibres that will create pores in the membrane

d. Lead carbide fibres that will create pores in the membrane

15. Use of hypodermic syringe to insert DNA into growing inflorescence is known as

a. Macroinjection

b. Micromanipulator mediated DNA delivery

c. Microfection

d. Microinjection

Answer Key

MCQ on Gene Transfer Methods

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