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Molecular Biology Techniques MCQs

MCQ on Molecular Biology Techniques; below can help students evaluate their knowledge of concepts. Answers are also given to help you remember. Try our MCQ on molecular biology techniques to see if you can get all the MCQ on molecular biology techniques answers right for the questions below.

Molecular Biology Techniques MCQs

Molecular Biology Techniques are used to investigate the molecular basis of biological activity. Protein methods, immunostaining, and nucleic acid methods are the most commonly used. These methods are used to investigate cells, their characteristics, parts, and chemical processes, focusing on how molecules control a cell’s activities and growth.

1. The method used to identify specific DNA sequences in bacterial colonies is known as

a. Colony Hybridization

b. In-Situ Hybridization

c. Dot Blot Technique

d. Western Blotting

2. Sothern hybridization is

a. Used to identify a specific protein

b. Used to identify a specific DNA

c. Used to identify a specific RNA

d. Used to identify both DNA and RNA

3. Which of the following statements about southern blotting is correct?

a. Developed by E.M.Southern

b. DNA-DNA hybridization is the basis

c. Transfer of DNA fragments from gel to the membrane is called blotting

d. All of these

4. Southern blotting has a variety of applications include?

a. DNA fingerprinting

b. Preparation of RFLP maps

c. Identification of transferred genes

d. All of these

5. The method for detecting the presence of DNA or RNA in a non-fractionated DNA sample is known as

a. Colony Hybridization

b. In Situ Hybridization

c. Dot Blot Technique

d. Western Blotting

6. Northern hybridization is

a. Used to identify a specific protein

b. Used to identify a specific DNA

c. Used to identify a specific RNA

d. Used to identify both DNA and RNA

7. In Northern hybridization probe hybridization forms

a. DNA: DNA hybrid

b. RNA: DNA hybrid

c. Both a and b

d. None of these

8. Except for one, there are no differences in procedure between Northern and Southern hybridization.

a. DBM membrane is used in northern hybridization

b. RNA: DNA hybrids are formed in northern hybridization

c. Initially fragments are separated by electrophoresis in northern hybridization

d. DNA denaturation is required before blotting in Sothern hybridization

9. Southern hybridization is

a. Used to identify a specific protein

b. Used to identify a specific DNA

c. Used to identify a specific RNA

d. Used to identify both DNA and RNA

10. In Western blotting

a. Agarose gel is commonly used

b. Polyacrylamide gel is commonly used

c. Both a and b

d. High-resolution gels

11. For glycoproteins, the most commonly used probe is

a. Antibody

b. Lectin

c. Antigens

d. Interferon

12. For protein detection, the most commonly used probe is

a. Antibody

b. Lectin

c. Antigens

d. Interferons

13. ELISA is based on

a. Antigen-antibody interaction

b. Antigen-protein interaction

c. Lectin- antibody interaction

d. All of these

14. The method for locating specific genes in chromosomes is known as

a. Colony hybridization

b. In situ hybridization

c. Dot blot technique

d. Western blotting

15. The separation of charged molecules under the influence of an electric current is known as

a. Colony hybridization

b. Electrophoresis

c. Dot blot technique

d. Western blotting

Answer Key

MCQ on Molecular Biology Techniques

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