MCQ on PCR (Polymerase Chain Reaction)

MCQ on Polymerase Chain Reaction post focuses on MCQ on PCR, history, enzymes, techniques and importance. Try our PCR practice questions, answers given below for your reference.

MCQ on PCR

MCQ on PCR

1. The PCR technique was developed by

a) Kary Mullis

b) Kohler

c) Milstein

d) Altman

2. Polymerase Chain Reaction is a

a) DNA degradation technique

b) DNA amplification technique

c) DNA sequencing technique

d) All of these

3. Which of the following statements about PCR is true?

a) From micrograms of DNA, billions of copies can be generated

b) Thermal cyclers are automated PCR devices.

c) A thermostable DNA Polymerase is required

d) All of these

4. Thermus aquaticus is the source of

a) Primase enzyme

b) Taq Polymerase

c) Vent Polymerase

d) Both 2 and 3

5. Which of the following is not a thermostable polymerase?

a) pfu polymerase

b) Taq polymerase

c) Vent polymerase

d) DNA polymerase III

6. Polymerase used for PCR is extracted from?

a) Homo sapiens

b) Thermus aquaticus

c) Escherichia coli

d) Saccharomyces cerevisiae

7. Which of the following is not an application of PCR

a) Molecular diagnosis

b) Gene amplification

c) Purification of isolated protein

d) Detection of gene mutation

8. Which of the following is not required for PCR?

a) DNA primer

b) DNA template

c) RNA primer

d) Taq polymerase

9. Taq polymerase is used in PCR because?

a) Heat-stable

b) Buffering

c) Denaturant

d) Large

10. Enzyme required for polymerase chain reaction (PCR) is

a) Taq polymerase

b) Ribonuclease

c) Endonuclease

d) RNA polymerase

11. Thermus aquaticus is the source of

a) Taq polymerase

b) Vent polymerase

c) Both a and b

d) Primase enzyme

12. Gene amplification using primers can be done by?

a) Microinjection

b) ELISA

c) Polymerase chain reaction

d) Gene gun

MCQ on PCR Answers

1. a) Kary Mullis

2. d) all of these

3. d) All of these

4. d) Both 2 and 3

5. d) DNA polymerase III

6. b) Thermus aquaticus

7. c) Purification of isolated protein

8. a) DNA primer

9. a) Heat-stable

10. a) Taq polymerase

11. a) Taq polymerase

12. c) Polymerase chain reaction

We hope PCR quiz questions helped you a lot. You can learn more about PCR and functions at NotesHippo.com

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