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MCQ on PCR Technique

MCQ on PCR Technique can help students evaluate their knowledge of concepts on genetic engineering and enzymes used in GE. Answers are also given to help you remember. Try our MCQ on PCR Technique to see if you can get all the MCQ on PCR Technique answers right for the questions below.

MCQ on PCR technique

The polymerase chain reaction (PCR) is a technique for amplifying DNA sequences in the lab. The process includes selecting the part of the genome to be amplified using short DNA sequences called primers.

1. The PCR method was created by

a. Kary Mullis

b. Kohler

c. Milstein

d. Altman

2. PCR is a

a. DNA degradation technique

b. DNA amplification technique

c. DNA sequencing technique

d. All of these

3. Which of the following statements about PCR is correct?

a. Billions of copies of desired DNA can be synthesized

b. Automated PCR machines are called thermal cyclers

c. A thermo stable DNA polymerase is required

d. All of these

4. Thermus aquaticus is the source of

a. Taq polymerase

b. Vent polymerase

c. Both a and b

d. Primase enzyme

5. Except for one, all of the following polymerases are thermostable?

a. Taq polymerase

b. Vent polymerase

c. DNA polymerase III

d. pfu polymerase

6. The basic needs for a PCR reaction include?

a. DNA segment to be amplified

b. Two oligonucleotide primers

c. A heat stable DNA polymerase

d. All of these

7. Because of this, Pfu and Vent polymerase are more efficient than Taq polymerase?

a. Of more efficient polymerase activity

b. Of proof reading activity

c. Both a and b

d. None of these

8. The first step of PCR is

a. Denaturation

b. Annealing

c. Primer extension

d. None of these

9. Denaturation involves

a. Heating between 90-980C

b. Heating between 40-600C

c. Heating between 720C

d. None of these

10. The process of primer binding to the denatured strand is known as?

a. Denaturation

b. Annealing

c. Renaturation

d. None of these

11. Which of the following statements about PCR is correct?

a. Denaturation involves heating at 90-980C

b. Annealing involves binding of primer between 40-600C

c. Primer extension occurs at 720C

d. All of these

12. Asymmetric PCR is

a. Used to generate single stranded copies for DNA sequencing

b. Used to generate double stranded copies for DNA sequencing

c. Both a and b

d. None of these

13. PCR is used in

a. Site specific recombination

b. Site directed mutagenesis

c. Both a and b

d. Site specific translocation

14. Reverse transcriptase PCR uses

a. mRNA as a template to from cDNA

b. RNA as a template to from DNA

c. DNA as a template to from ssDNA

d. All of these

15. PCR is used to

a. Amplify gene of interest

b. Construct RAPD maps

c. Detect the presence of transgene in an organism

d. All of these

Answer Key

MCQ on PCR Technique

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