Recombinant DNA technology is a ground-breaking field that has revolutionized the world of biotechnology and genetics. MCQs are an effective way to test your knowledge and understanding of the key concepts in this field.
In this MCQ on Recombinant DNA Technology blog, we’ll be exploring some of the fundamental principles of recombinant DNA technology through a series of MCQs. Whether you’re a student, researcher, or just curious about this fascinating area of science, this MCQ on Recombinant DNA Technology blog will help you test your knowledge and deepen your understanding of the topic. So let’s dive in!
MCQ on Recombinant DNA Technology
1. Which enzyme is used to cut DNA at specific sequences in rDNA Technology?
A) RNA polymerase
B) DNA polymerase
C) Restriction endonucleases
D) Ligases
2. Which is not a part of the basic steps involved in rDNA Technology?
A) Restriction digestion of DNA
B) Ligation of DNA fragments
C) Transformation of host cells
D) Electrophoresis of proteins
3. Which of the following is not a commonly used vector in rDNA Technology?
A) Plasmids
B) Bacteriophages
C) Cosmids
D) Liposomes
4. Which of the following is used to amplify DNA in vitro during rDNA Technology?
A) Polymerase chain reaction
B) Southern blotting
C) Northern blotting
D) Western blotting
5. Which is an example of a selectable marker used in rDNA Technology?
A) GFP
B) β-galactosidase
C) Antibiotic resistance gene
D) Ampicillin
6. Which is used to visualize DNA fragments in electrophoresis?
A) Ethidium bromide
B) Coomassie Blue
C) Silver stain
D) Hematoxylin
7.Which is used to insert foreign DNA into host cells during rDNA Technology?
A) Electroporation
B) Sonication
C) Chromatography
D) Gel electrophoresis
8. Which of the following is used to identify recombinant clones in rDNA?
A) Restriction enzymes
B) PCR
C) Southern blotting
D) Western blotting
9. Which of the following is used to detect the presence of specific DNA sequences in rDNA Technology?
A) PCR
B) Electrophoresis
C) Transformation
D) Ligation
10. Which of the following is used to purify DNA fragments in rDNA Technology?
A) Gel electrophoresis
B) Chromatography
C) Sonication
D) Restriction enzymes
11. Which of the following is a common host cell used in rDNA Technology?
A) E. coli
B) Yeast
C) Mammalian cells
D) All of the above
12. Which of the following is a commonly used promoter in rDNA Technology?
A) T7 promoter
B) lac promoter
C) trp promoter
D) all of the above
13. Which of the following is used to introduce DNA into plant cells during RDNA Technology?
A) Agrobacterium
B) Electroporation
C) Sonication
D) Gel electrophoresis
14. Which following is used to create a fusion protein in rDNA Technology?
A) In vitro mutagenesis
B) Site-directed mutagenesis
C) PCR
D) Cloning
15. Which of the following is not a type of restriction endonuclease?
a) EcoRI
b) BamHI
c) Taq polymerase
d) HindIII
Answers
1. C) Restriction endonucleases
2. D) Electrophoresis of proteins
3. D) Liposomes
4. A) Polymerase chain reaction
5. C) Antibiotic resistance gene
6. A) Ethidium bromide
7. A) Electroporation
8. A) Restriction enzymes
9. A) Polymerase chain reaction
10. B) Chromatography
11. D) All of the above
12. D) all of the above
13. A) Agrobacterium
14. D) Cloning
15. c) Taq polymerase