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Restriction Enzymes MCQ Questions

Restriction Enzymes MCQ Questions can help students evaluate their knowledge of concepts on genetic engineering and enzymes used in GE. Answers are also given for MCQ on restriction enzymes to help you remember. Try our MCQ on restriction enzymes to see if you can get all the answers right for the questions below.

Restriction Enzymes MCQ Questions

Genetic engineering is the process of altering an organism’s genetic makeup using recombinant DNA (rDNA) technology. The alteration of one or more genes is referred to as genetic engineering. To give an organism a desirable phenotype, a gene from another species is usually inserted to its genome.

MCQ on Restriction Enzymes

1. Restriction enzymes HaeIII and AluI cut right across the double helix to produce?

a. Sticky Ends

b. Blunt Ends

c. Codons

d. Anticodons

2. Zinc-finger nucleases is

a. Natural restriction enzyme

b. Type of polymerase enzyme

c. Artificial restriction enzyme

d. Type of helicase enzyme

3. Sticky ends are overhanging pieces of single stranded

a. DNA

b. RNA

c. Proteins

d. Messenger RNA

4. Restriction enzymes are also called

a. Restriction sites

b. Restriction endonucleases

c. Restriction polymerases

d. Endonucleases

5. Who discovered restriction enzymes?

a. Watson and Crick

b. Jacob and Monad

c. Nathan, Arber and Smith

d. Boyer and Cohen

6. Restriction enzymes are enzymes

a. Capable of cutting DNA molecule

b. Capable of adding nucleotides to the 3’OH end

c. Capable of restricting protein synthesise

d. Capable of joining DNA molecules

7. Restriction enzymes can make internal cuts in a DNA molecule called?

a. Restriction exonuclease

b. Restriction endonuclease

c. Both a and b

d. S1 nuclease

8. Restriction enzymes are also called

a. Molecular knives

b. Molecular scissors

c. Molecular scalpels

d. All of these

9. Restriction enzyme recognises a certain sequence to cut the DNA is known as?

a. Recognition site

b. Restriction site

c. Both a and b

d. Cleavage sites

10. Which of the following statements about restriction enzyme is correct?

a. Restriction enzyme are used to cut DNA molecule

b. Restriction enzyme are used to construct restriction maps

c. Restriction enzyme are used in RFLP

d. All of these

11. The type of restriction enzymes used in rDNA technology is

a. Type I

b. Type II

c. Type III

d. All of these

12. Which of the following claims about restriction enzymes is correct?

a. Type I and II enzymes cuts far away from the restriction sites

b. Type II cuts DNA within restriction sites

c. Eco R1 is a Type II restriction enzyme

d. All of these

13. Restriction sites of type II enzymes

a. Generally are palindromic sequences

b. Consists of 4-6 bp

c. Mostly are palindromes with rotational symmetry

d. All of these

14. RE forms single-stranded unpaired extensions when it cleaves a DNA is called?

a. Blunt ends

b. Flush ends

c. Sticky ends

d. None of these

15. Type II restriction enzymes require which of the following ions to function?

a. Ca2+

b. Mg2+

c. Cl2+

d. Mn2+

Answer Key

MCQ on Restriction Enzymes

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