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Thin Layer Chromatography MCQ with Answers

Thin Layer Chromatography MCQ with answers can help students evaluate their knowledge of concepts in Thin Layer Chromatography. Answers are also given to help you remember. Try our Thin Layer Chromatography MCQ to see if you can get all the answers right for the questions below.

Thin Layer Chromatography is a method for isolating non-volatile mixtures. The experiment is carried out on a sheet of aluminium foil, plastic, or glass that has been coated with a thin layer of adsorbent material.  Aluminium oxide, cellulose, or silica gel is the most common materials used.

Thin Layer Chromatography MCQ

1. What is the principle of TLC?

  1. Partition
  2. Absorption
  3. Adsorption
  4. A and B

2. Thin layer chromatography is

  1. Partition chromatography
  2. Electrical mobility of ionic species
  3. Adsorption chromatography
  4. None of the above

3. In TLC, initially the sample is

  1. In contact with mobile phase
  2. Not in contact with mobile phase
  3. Coated at the level of mobile phase
  4. Coated below the level of mobile phase

4. Which method is used to identify spots on the TLC plate?

  1. Spraying with reagents
  2. Under microscope
  3. Fluorescence
  4. Fluorescent adsorbent

5. Except one, all of the following are used as spraying reagents in TLC.

  1. Calcium sulfate
  2. Iodine
  3. Sulfuric acid
  4. Ninhydrin

6. Which one can be used to make the mobile phase in chromatography?

  1. Solid or liquid
  2. Liquid or gas
  3. Gas only
  4. Liquid only

7. Which one can be used to make the mobile phase in chromatography?

  1. Solid or liquid
  2. Liquid or gas
  3. Gas only
  4. Liquid only

8. In chromatography, the stationary phase can be ___ supported on a solid.

  1. Solid or liquid
  2. Liquid or gas
  3. Solid only
  4. Liquid only

9. Chromatography is a physical method for separating and analyzing?

  1. Simple mixtures
  2. Complex mixtures
  3. Viscous mixtures
  4. Metals

10. Components with a lower affinity for stationary phases are

  1. Travels slowly
  2. Travels fast
  3. A and B
  4. None of the above

11. The components that have a stronger affinity for stationary phases are

  1. Travels slowly
  2. Travels fast
  3. A and B
  4. None of the above

12. Which is not a consideration when selecting an adsorbent?

  1. Nature of the adsorbent
  2. Solubility of the adsorbent
  3. Chemical nature
  4. Temperature

13. Which sentence is false about TLC ?

  1. It is very sample method
  2. It is less time consuming.
  3. Applied to allow type of sample.
  4. It is very costly.

14. In TLC, initially the sample is

  1. In contact with mobile phase
  2. Not in contact with mobile phase
  3. Coated at the level of mobile phase
  4. Coated below the level of mobile phase

15. In thin layer chromatography, what is the stationary phase?

  1. Liquid held between glasses
  2. Glass plate
  3. Silica gel
  4. None of the above

Further Readings

Reference