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Cloning vector MCQ with answers
A cloning vector is a small piece of DNA that can be kept stable in an organism and into which a foreign DNA fragment can be inserted for cloning. The cloning vector could be DNA from a virus, a cell from a higher organism, or a bacterium’s plasmid.
Cloning Vectors MCQs
1. Maximum size of foreign DNA that can be inserted into a replacement vector is
a. 25-30 kb
b. 18-20 kb
c. 20-25 kb
d. 40-50 kb
2. pBR 322 has/have which of the following selection marker(s)?
a. Ampr
b. Tetr
c. Both (a) and (b)
d. Kanr
3. Difference between λ gt 10 and λ gt 11 vectors is that
a. λ gt 11 is an expression vector
b. λ gt 10 is an expression vector
c. λ gt 10 is a replacement vector
d. λ gt 11 is a replacement vector
5. Select the wrong statement about plasmids?
a. It is extrachromosomal
b. It is double-stranded
c. Its replication depends upon host cell
d. It is closed and circular DNA
6. Stuffer is
a. The right arm of the vector DNA
b. The left arm of the vector DNA
c. Central fragment of the vector DNA
d. None of the above
7. Conjugative plasmids
a. Exhibit antibiotic resistance
b. Do not exhibit antibiotic resistance
c. Carry transfer genes called the tra genes
d. Do not carry transfer genes
8. P1 cloning vector is the example of
a. Plasmid
b. Cosmid
c. Bacteriophage
d. Phagemid
9. Cos site of the cosmids
a. Consists of 12 bases
b. Helps whole genome in circularization and ligation
c. Both (a) and (b)
d. Contains cleavage site
10. Phagemid vectors are
a. Combination of plasmid and phage λ
b. Combination of phages and Cosmid
c. Phages carrying properties of plasmids
d. All of the above
11. Single stranded vectors are useful
a. For sequencing of cloned DNA
b. For oligonucleotide directed mutagenesis
c. For probe preparation
d. All of the above
12. Cosmid vectors are used for
a. Cloning small fragments of DNA
b. Cloning large fragments of DNA
c. Cloning prokaryotic DNA only
d. Cloning eukaryotic DNA only
13. Maximum size of foreign DNA that can be inserted into an insertion vector is
a. 35 kb
b. 18 kb
c. 50 kb
d. 27 kb
14. Plasmids which are maintained as multiple copy numbers per cell are known as
a. Stringent plasmids
b. Relaxed plasmids
c. Cryptic plasmids
d. None of these
15. Cosmid vectors are
a. Plasmids that contain a fragment of λ DNA including the cos site
b. Phages that lack cos site
c. Plasmids that have no selection marker
d. Cryptic plasmids
Answer Key
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