In this design and layout of animal tissue culture Laboratory post we have briefly explained about design and layout of culture room, requirements, animal tissue culture lab design, and animal tissue culture laboratory services.
Design and Layout
The need to maintain asepsis distinguishes the tissue culture laboratory from most others, so it is important it be dust free and have no through traffic. The introduction of laminar flow hoods has greatly simplified the problem and allows the utilization of unspecialized animal tissue culture laboratory accommodation, provided that the location is suitable.
Animal cell culture laboratory design depends on the type and scale of the operations and the number of users, but some general principles apply. Design and layout of culture room for easy cleaning. Furniture should fit tightly to the floor or be suspended from the bench with a space left underneath for cleaning.
Cover the floor with a coved vinyl, acrylic coating, or other dustproof finish, and allow a slight fall in the level toward a floor drain located outside the door of the room. This arrangement allows liberal use of water if the floor has to be washed, but more important, it protects equipment from damaging floods if stills, autoclaves, or sinks overflow.
Figure 1: Design and Layout of Animal Tissue Culture Laboratory
Provision must be made for preparation and sterilization, aseptic handling, other activities within the culture area including centrifugation, cell counting, microscopy, incubation, and storage at room temperature, 4⁰C, −20⁰C, and −196⁰C. Number of users determine how many laminar-flow hoods will be required (based on whether people can share hoods or will require a hood each for most of the day) and whether a large area will be needed to handle bioreactors, animal tissue dissections, or large numbers of cultures.
The largest area should be given to the culture operation, which has to accommodate laminar-flow hoods, cell counters, centrifuges, incubators, microscopes, and using stocks of reagents, media, glassware, and plastics, and, if possible, a quarantine area in design and layout of culture room. The second largest is for wash up, preparation, and sterilization, the third is for storage, and the fourth is for incubation.
c. Preparation Area
Facilities for washing up and for sterilization should be located (a) close to the aseptic area that they service and (b) on an outside wall to allow for the possibility of heat extraction from ovens and steam vents from autoclaves in design and layout of culture room.
What is the scale of the work contemplated and how much storage space will this require for disposable plastics, and so on? What proportion of the work will be cell line work, with its requirement for storage in liquid nitrogen.
Make sure that doorways are wide enough and high enough and that ceilings have sufficient clearance to allow the installation of equipment such as laminar-flow hoods, incubators, and autoclaves. Provide space for access for maintenance of equipment.
This is one of the more difficult problems because containment requires that the contents of the tissue culture room not escape to adjacent work areas, while asepsis requires that none of the contamination of surrounding areas enters the tissue culture. It is essential to have a dedicated tissue animal tissue culture lab design with an adjacent preparation area, or a number of smaller ones with a common preparation area, rather than to have tissue culture performed alongside regular laboratory work with only a laminar-flow hood for protection. A separate facility gives better contamination protection, allows tissue culture stocks to be kept separate from regular laboratory reagents and glassware, and will, in any case, be required for containment if human or other primate cells are handled.
Culture Lab Services
Hot and cold water, power, combustible gas (domestic methane, propane, etc.), carbon dioxide, and compressed air will be required. Power is always underestimated, in terms of both the number of outlets and the amperage per outlet. Hot and cold water with sinks and drainage will be required in both the preparation and the tissue culture areas. Adequate floor drainage should be provided in the preparation/wash up area, with a slight fall in floor level from the tissue culture lab to the wash up. A vacuum line can be useful for evacuating culture flasks, but a collection vessel must be present with an additional trap flask, with a hydrophobic filter between the flasks, in order to prevent fluid, vapor, or some contaminant from entering the vacuum line and pump.