1. 40% Acrylamide: Acrylamide 116.8 g, N,N’-Methylene bisacrylamide 3.2 g, DD H2O to 300 ml.
2. 30% Ammonium Persulfate: Ammonium Persulfate 1.5 g, DD H2O 5ml (Store at 4°C. Replace every month).
3. 1.5 M Tris HCl, pH 8.8: DD H2O 300 ml, Tris–free base 90.75 g, Conc. HCl 8 ml (Adjust to pH 8.8 with conc. HCl, and bring final volume to 500 ml with DD H2O).
4. 1.0 M Tris HCl, pH 6.8: DD H2O 300 ml, Tris–free base 60.54 g Conc. HCl 36 ml (Adjust to pH 6.8 with conc. HCl, and bring final volume to 500 ml with DD H2O).
5. 4x SDS-PAGE Sample Buffer: 125 mM Tris HCl, pH 6.8 1 M 5 ml, 20% Glycerol 8 ml, 4% SDS 20% 8 ml, 10% ß-Mercaptoethanol 4 ml, 0.5 mg/ml Bromophenol Blue 20 mg, DD H2O 15 ml.
6. 10x SDS-PAGE Running Buffer: 30.3 g Tris base, 144.0 g Glycine, 10.0 g SDS (Dissolve and bring total volume to 1,000 ml with deionized water. Do not adjust pH with acid or base (pH is normally 8.3 as prepared).
7. Coomassie Stain Solution: Ethanol 150 ml, Glacial Acetic Acid 50 ml, DD H2O 300 ml, Coomassie Brilliant Blue–R-250 1 g (Dissolve Coomasie Brilliant Blue–R-250 in EtOH first).
8. Destain Solution: Ethanol 1200 ml, Glacial Acetic Acid 400 ml, DD H2O 2.4 l ((pH is neutral as prepared).