TBS Tris Buffered Saline 10X Solution pH 7.4

TBS Tris buffered saline 10X solution pH 7.4 post we have briefly explained about 10X and 20X TBS and TBST buffer composition.

TBS Tris Buffered Saline 10X Solution pH 7.4

Tris Buffered Saline (TBS) is an isotonic pH stabilising buffer solution often used in immunoassay processes to provide optimal antibody-antigen binding interactions. It’s used in immunohistochemistry as a substance diluent for washes between sequential immunoreactions to eliminate unbound immunological agents, as well as incubation mixtures for labelled antibodies and other immunoreagents to reduce unspecific background noise. Tris Buffered Saline buffer is a key component of the buffers used in the washing, blocking, and antibody dilution operations in Western blotting.

Tris Buffered Saline is non-toxic and isotonic. It’s useful for diluting chemicals used in lab research. Additives can improve the functionality of a chemical. In immunoblotting, TBS is frequently used for both membrane washing and antibody dilution.

Tris Buffered Saline (TBS)

1. 10X Tris Buffered Saline (TBS) (0.5M Tris Base, 9% NaCl, pH 7.4)

Trizma base: 61 g

NaCl: 90 g

Distilled water: 1000 ml

Mix to dissolve and adjust pH to 7.4 using concentrated HCl. Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

2. 20X TBS (1M Tris Base, 18% NaCl, pH 7.4)

Trizma base: 122 g

NaCl: 180 g

Distilled water: 1000 ml

Mix to dissolve and adjust pH to 7.4 using concentrated HCl. Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

3. 10X TBS-Tween 20 (0.5M Tris Base, 9% NaCl, 0.5% Tween 20, pH 8.4)

Trizma base: 61 g

NaCl: 90 g

Distilled water: 1000 ml

Mix to dissolve and adjust pH to 8.4 using concentrated HCl and then add 5 ml Tween 20. Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

4. 20X TBS-Tween 20 (1M Tris Base, 18% NaCl, 1% Tween 20, pH 8.4)

Trizma base: 122 g

NaCl: 180 g

Distilled water: 1000 ml

Mix to dissolve and adjust pH to 8.4 using concentrated HCl and then add 10 ml Tween 20. Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

Further Readings

Reference

  1. https://www.bosterbio.com/media/pdf/AR0031_DS.pdf
  2. http://www.medicago.se/tris-buffered-saline-tbs-ph-80-and-76
  3. https://en.wikipedia.org/wiki/Tris-buffered_saline