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Tris HCL Buffer Recipe pH 7.6

Tris HCL Buffer Recipe pH 7.6 post we have briefly explained about 10X and 20X Tris HCL and Tris HCL Tween-20 buffered saline preparation protocol.

Tris HCL Buffer Preparation

The difference between tris and tris HCl is that tris is a basic buffer and tris HCL buffer is an acidic buffer. Buffers are used to protect against pH shifts. Without a buffer, even modest quantities of a strong acid or base can drastically alter the pH of the environment. As a result, the decision between tris HCL buffer and tris base may be influenced by what you want to accomplish with the buffer.

It is a buffering agent (acidic buffer) that is often employed by molecular biologists to modify or maintain the pH of a solution. It can be used in a variety of buffer formulations, including:

CTAB DNA extraction buffer

Leammli buffer for SDS-PAGE

TAE buffer

TBE buffer

TE buffer

The protocol you’re working with will determine whether you use tris HCL buffer or tris base for these typical recipes. You can utilise the conjugate acidic or basic component to modify the pH of your solution.

10X Tris-HCl (0.5M Tris Base, pH7.6)

Trizma Base: 61 g

Distilled water: 1000 ml

Adjust pH 7.6 using concentrated HCl. Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

20X Tris-HCl (1M Tris Base, pH7.6)

Trizma Base: 122 g

Distilled water: 1000 ml

Adjust pH 7.6 using concentrated HCl. Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

10X Tris-HCl-Tween 20 (0.5M Tris Base, 0.5% Tween 20, pH7.6)

Trizma Base: 61 g

Distilled water: 1000 ml

Adjust pH 7.6 using concentrated HCl and then add 5 ml of Tween 20. Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

Further Readings

Reference