What is the difference between nitrocellulose and PVDF membranes

What is the difference between nitrocellulose and PVDF membranes post briefly explains about nitrocellulose membranes, PVDF membranes, nitrocellulose membrane vs PVDF.

1. What is Nitrocellulose Membrane?

2. What is PVDF Membrane?

3. Nitrocellulose membrane vs PVDF

The capacity of nitrocellulose membrane to bind proteins is significantly higher than that of PVDF membrane, which has a very low protein-binding capacity in comparison. This is the critical difference between nitrocellulose membrane vs PVDF membranes.

The binding of protein molecules to the nitrocellulose membrane happens by hydrophobic interactions, whereas binding of protein molecules to the PVDF membrane occurs via both hydrophobic and dipole interactions. The sensitivity of the nitrocellulose membrane is significantly greater than that of the PVDF membrane.

Western blot uses nitrocellulose membranes and PVDF membranes to transfer proteins that have been separated by gel electrophoresis. Membranes in blotting serve primarily as a physical structure to hold protein fragments together.

What is Nitrocellulose Membrane?

When it comes to western blotting, nitrocellulose membrane was one of the original types of membranes that was used. It is both instantaneous and almost completely irreversible; it continues to be the option of choice for this method.

In addition to this, the nitrocellulose membrane readily absorbs water. However, you cannot strip it, reprobe it, or subject it to harsh chemical treatments using this material. The inert support structure of supported nitrocellulose membranes increases the strength and durability of the membrane.

Electrophoretic transfer is another technique used in western blotting that transfers gel-bound protein fragments to nitrocellulose membrane. The idea behind this process is that the negatively charged proteins move toward the anode.

What is the difference between nitrocellulose and PVDF membranes

Western Blotting Transfer Method

What is PVDF Membrane?

The other kind of membrane that can be used in western blotting is called a PVDF membrane. In comparison to the nitrocellulose membrane, it has a greater capability for binding proteins. Consequently, this results in an improvement in the detection’s sensitivity as well.

It is also important to note that the PVDF membrane does not need to be pre-wetted prior to usage. This prevents the precipitation of high molecular weight proteins by low SDS/high methanol solutions. In PVDF membranes, having SDS in the transfer buffer makes blotting work better.

Now let’s take a brief look at nitrocellulose membrane vs PVDF differences.

What is the difference between nitrocellulose and PVDF membranes

Nitrocellulose membrane vs PVDF. Image Source: Detection of Blotted Proteins

Difference between Nitrocellulose and PVDF Membranes

Characteristics

The nitrocellulose membrane is extremely brittle and fragile, in contrast to the PVDF membrane, which is physically resilient.

Resistant

The PVDF membrane offers chemical resistance, in contrast to the lack of such properties exhibited by the nitrocellulose membrane.

Pre-Wetting

While the nitrocellulose membrane must be pre-wetted with methanol, the PVDF membrane does not need to be pre-wetted before use.

Capacity

Nitrocellulose membrane has a protein binding capacity is relatively modest in contrast to the PVDF membrane, that has a good protein binding capacity.

Interactions

Proteins connect to nitrocellulose membranes by hydrophobic interactions, whereas PVDF membranes allow for protein binding via both hydrophobic and dipole interactions.

Sensitivity

The sensitivity of the nitrocellulose membrane is relatively low in comparison to that of the PVDF membrane, which has a high binding capacity and therefore a high level of sensitivity.

Background

Nitrocellulose membrane has a lesser background due to its lower sensitivity, while PVDF membrane has a stronger background due to its higher sensitivity.

Usage

Nitrocellulose membrane is utilised for the separation of proteins with low molecular weights, while PVDF membrane is utilised for the separation of proteins with high molecular weights.

Reprobing

PVDF membranes are easier to strip and reprobe than nitrocellulose membranes because of their hydrophobicity.

Other Uses

Nitrocellulose membrane is employed for the analysis of nucleic acids that are less than 300 base pairs (bp), amino acid analysis, and dot/slot blotting. PVDF membrane, on the other hand, is utilised for proteins sequence, amino acid analyzation, and solid-phase assay systems.

Nitrocellulose Membrane vs PVDF

Nitrocellulose Membrane vs PVDF

The answer to the question of nitrocellulose membrane vs PVDF has been found. You can learn more about bioinstrumentation and techniques at NotesHippo.com

Use our password generator tool to make a strong password. Get a strong passwords today at www.passwrdgenerator.com to help stop a security threat.